Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod
Pearman, W. S.; Wells, Sarah; Dale, J.; Silander, O. K.; Freed, N. E.
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Citation:Pearman, W. S., Wells, S. J., Dale, J., Silander, O. K., & Freed, N. E. (2022). Long-read sequencing reveals atypical mitochondrial genome structure in a New Zealand marine isopod. Royal Society Open Science, 9, 211550. doi:10.1098/rsos.211550
Permanent link to Research Bank record:https://hdl.handle.net/10652/5678
Most animal mitochondrial genomes are small, circular and structurally conserved. However, recent work indicates that diverse taxa possess unusual mitochondrial genomes. In Isopoda, species in multiple lineages have atypical and rearranged mitochondrial genomes. However, more species of this speciose taxon need to be evaluated to understand the evolutionary origins of atypical mitochondrial genomes in this group. In this study, we report the presence of an atypical mitochondrial structure in the New Zealand endemic marine isopod, Isocladus armatus. Data from long- and short-read DNA sequencing suggest that I. armatus has two mitochondrial chromosomes. The first chromosome consists of two mitochondrial genomes that have been inverted and fused together in a circular form, and the second chromosome consists of a single mitochondrial genome in a linearized form. This atypical mitochondrial structure has been detected in other isopod lineages, and our data from an additional divergent isopod lineage (Sphaeromatidae) lends support to the hypothesis that atypical structure evolved early in the evolution of Isopoda. Additionally, we find that an asymmetrical site previously observed across many species within Isopoda is absent in I. armatus, but confirm the presence of two asymmetrical sites recently reported in two other isopod species
Keywords:New Zealand, Isocladus armatus, marine isopoda, genomics, evolutionary genetics, genotyping by sequencing (GBS), isolation-by-adaptation, isolation-by-distance, population genomics, isopods, RADseq
ANZSRC Field of Research:3104 Evolutionary biology, 3105 Genetics
Copyright Holder:© 2022 The Authors. Published by the Royal Society.
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